ps6 rb  (Cell Signaling Technology Inc)

Journal: Proceedings. Biological sciences

Article Title: Behavioural and neural correlates of social hierarchy formation in a sex-changing fish.

doi: 10.1098/rspb.2024.2097

Figure Lengend Snippet: Figure 3. Neural activation patterns after social disruption. Representative images for each brain area are depicted on the left (i; rank 2) and middle (ii; ranks 3−5) in each panel. Boxplots of pS6 counts are depicted on the right of each panel, with 2c and 3−5c representing the controls (iii). (A) The medial part of the dorsal telencephalon (Dm), with magnification of the insets to the right of each panel (scale bar of insets = 20 µm). (B) The dorsal part of the ventral telencephalon (Vd). (C) The ventral part of the ventral telencephalon (Vv). (D) The supracommissural nucleus of the ventral telencephalon (Vs). (E) The central nucleus of the telencephalic area (Vc). (F) the preoptic area (POA). (G) the ventral tuberal region of the hypothalamus (vTn). (H) the periaqueductal gray (PAG). (I) the periventricular nucleus of the posterior tuberculum (TPp). (J) The anterior tuberal nucleus (aTn; figure 4J). Scale bar = 100 µm. (K) Overview of brain regions with significantly different pS6 counts in rank 2 fish compared to other ranks.

Article Snippet: Non-specific binding was blocked (1% bovine serum albumin, 0.3% Triton-X and 5.0% normal goat serum made in 1 × PBS) for 2 h at room temperature, and slides were incubated with primary pS6 antibody (1 : 1500; Cell Signaling Technologies pS6 ribosomal protein S235/236 rabbit monoclonal antibody no. 4858) overnight at 4°C.

Techniques: Activation Assay, Disruption